DREADD-based manipulation of hippocampal astrocyte Gq signalling in a chronic mouse model of medial temporal lobe epilepsy
Dimitri De Bundel, Yana Van Den Herrewegen, Surajit Sahu, Marcus Dyer, Liam Nestor, Ann Van Eeckaut, Ilse Smolders · FENS Forum 2024
Medial temporal lobe epilepsy (MTLE) is a common cause of treatment-resistant seizures where excessive and hyper-synchronized neuronal activity is associated with reactive astrogliosis. Emerging evidence has indicated a role for neuron-astrocyte interactions in seizure activity. Previous studies have found increased astrocyte Ca2+ signals in mouse models for MTLE, but it has been difficult to disentangle how these drive or mitigate seizure activity in chronic epilepsy. We used an AAV8-GFAP-hM3Dq-mCherry vector to manipulate reactive astrocytes in the chronic phase of the mouse intrahippocampal
ePoster · Neuroscience