Nanoscopic distribution of VAMP2 and VAMP7 in striatal cholinergic varicosities and their respective localization with VAChT and VGLUT3 in synaptic vesicles
Mazarine Desplanque, Paola Cristofari, Odile Poirel, Alison Hébert, Sylvie Dumas, Véronique Fabre, Stéphanie Daumas, Nicolas Pietrancosta, Salah El Mestikawy, Véronique Bernard · FENS Forum 2024
AimStriatal cholinergic interneurons (CINs) use both acetylcholine (ACh) and glutamate (Glu) to regulate the striatal network. CINs express vesicular transporters for acetylcholine (VAChT) and Glu (VGLUT3). We recently demonstrated, using super resolution STimulated Emission Depletion microscopy (STED) microscopy, that part of cholinergic synaptic vesicles (SVs) contains both VAChT and VGLUT3, whereas others express only VAChT or only VGLUT3. CINs SVs are thus able to release either ACh or Glu or both. However, the link between neurotransmitters accumulation into SVs and their final exocytosis
ePoster · Neuroscience