Chronic visualization of microcirculation in mice using viral vectors expressing fluorescent protein-fused albumin
Xiaowen Wang, Marta Vittani, Christine Delle, Antonis Asiminas, Philip Alexander Gade Knak, Ayumu Konno, Masahiro Fukuda, Hirokazu Hirai, Maiken Nedergaard, Hajime Hirase · FENS Forum 2024
Studying blood microcirculation is crucial for understanding vascular diseases, yet current imaging methods involving acute administration of fluorescent dyes are invasive and short-lived. To address this limitation, we present here two novel approaches for longitudinal vasculature study. Given that albumin constitutes ~60% of plasma protein and is produced by liver hepatocytes, we developed adeno-associated viral vectors (AAVs) with the hepatocyte-specific P3 optimized transthyretin promoter to express the albumin-mNeonGreen or albumin-mScarlet fusion protein. These AAVs were packaged wit
ePoster · Neuroscience