Topic: anti-ALFA nanobodies

α2δ proteins serve as auxiliary subunits of voltage-gated calcium channels (CaV) and regulate the formation and differentiation of synapses. To comparatively study the neuronal localization of the four α2δ isoforms (α2δ-1 to -4), we previously established hemagglutinin (HA) tagging, which allows the surface detection with anti-HA antibody labeling. However, localizing α2δ proteins in synaptic membranes is limited because the synaptic cleft is densely packed with trans-synaptic and extracellular matrix proteins. Thus, classical antibodies have restricted access to extracellular epitopes. Here,