Topic: anti-GluN1 antibodies

The anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis is an autoimmune disorder characterized by specific autoantibodies targeting the GluN1 subunit of postsynaptic NMDARs. Here, we investigated the effects of patient monoclonal anti-GluN1 antibodies on the synaptic signalling and plasticity molecules CaMKII and DAPK1 and their related pathways.We incubated primary hippocampal cell cultures (div 14-21) with patient-derived monoclonal anti-GluN1 or control antibodies. Thereafter, we induced chemical LTP and/or treated cells with the DAPK1 inhibitor TC-DAPK6. We then analysed NMDARs GluN1