Topic: Argonaute-2 (Ago2)

Regulation of local protein synthesis is a critical aspect of synaptic plasticity and memory formation. Dendritic protein synthesis can be rapidly downregulated by microRNAs in conjunction with the RNA-induced silencing complex (RISC), of which Argonaute-2 (Ago2) is the core component. We previously demonstrated that NMDAR stimulation in vitro induces Ago2 phosphorylation at S387, which downregulates Limk1 expression via miR-134 as a result of increased binding of phosphorylated Ago2 to Limk1 mRNA. To explore the role of Ago2 S387 phosphorylation in vivo, we generated an Ago2S387A/S387A (phosp