Transcriptomic characterization of maturing neurons from human neural stem cells across developmental time points and their application in developmental neurotoxicity screening
Kimia Hosseini, Gaëtan Philippot, Sara Salomonsson, Andrea Cediel-Ulloa, Elnaz Gholizadeh, Anna Forsby, Robert Fredriksson · FENS Forum 2024
The Ion AmpliSeqTM was employed for the temporal transcriptomic characterization of the maturation of human stem cell derived neurons at multiple time points. Analysis of the data revealed a progressive increase in markers associated with neuronal development and astrocyte markers over the time. Indicating the establishment of a co-culture containing both glial cells and neurons, and highlighting the importance of culture duration. With the help of unbiased bioinformatics, the top 100 genes driving the principal component analysis clustering and the top 20 differentially expressed genes associ
ePoster · Neuroscience