Topic: ATP deprivation

During metabolic stress, such as ischemic stroke, insufficient energy supply leads to ATP deprivation, resulting in ion imbalances and depolarization of cells. This results in uncontrolled glutamate release and impaired uptake by astrocytes. However, the role of elevated extracellular glutamate concentrations in exacerbating metabolic stress remains unclear. To investigate glutamate dynamics in the extracellular space during the acute phase of energy deprivation, we utilized the green-fluorescent glutamate sensor SF-iGluSnFR1. Organotypic mouse brain slice cultures were transduced with synapsi