Altered cell membrane ganglioside composition affects enzyme activity, expression, and submembrane localization of Na+,K+-ATPase in mouse brain
Borna Puljko, Mario Stojanović, Katarina Ilić, Nikolina Maček Hrvat, Marta Balog, Marija Heffer, Svjetlana Kalanj Bognar, Kristina Mlinac Jerković · FENS Forum 2024
Gangliosides, as major lipid raft (LR) constituents, provide an optimal milieu for the function and activity of various membrane proteins. One example is the Na+,K+-ATPase (NKA) vital for cell ion homeostasis. Aiming to investigate effects of gangliosides on NKA gene and protein expression, enzyme activity, and submembrane localization, we analyzed cortices and cerebella derived from a mouse model with impaired synthesis of gangliosides (St8sia1 null) in comparison with wild type (WT) mice. Gene expression of six NKA isoforms was determined by qRT-PCR, while NKA protein expression was analyzed
ePoster · Neuroscience