Integrating network activity with transcriptomic profiling in hiPSCs-derived neuronal networks to understand the molecular drivers of functional heterogeneity in the context of neurodevelopmental disorders
Sofia Puvogel, Ummi Ciptasari, Eline van Hugte, Shan Wang, Nicky Scheefhals, Astrid Oudakker, Chantal Schoenmaker, Ka Man Wu, Hans van Bokhoven, Dirk Schubert, Nael Nadif Kasri · FENS Forum 2024
To understand the functional heterogeneity in neuronal activity associated with neurodevelopmental disorders (NDDs) and identify its molecular basis, we integrated RNA-sequencing and micro-electrode array (MEA) recordings in hiPSCs-derived neurons carrying NDD-related mutations. Clustering analysis of 345 recordings from 35 NDD-related cell lines at 28 days in-vitro (DIVs) revealed seven discrete activity phenotypes. While a continuous phenotypic landscape of network alterations emerged, specific clusters were identified for certain disorders. At DIV49, stronger associations between activity p
ePoster · Neuroscience