Loss of neuroprotection in Alzheimer’s disease: Shedding light on BDNF receptor cleavage and its mirroring in extracellular vesicles
Tiago Costa-Coelho, João Fonseca-Gomes, Gonçalo Garcia, Mafalda Ferreira-Manso, Catarina B. Ferreira, Carolina de Almeida-Borlido, Juzoh Umemori, Mikko Hiltunen, Eero Castrén, Ana M. Sebastião, Alexandre de Mendonça, Dora Brites, Maria José Diógenes · FENS Forum 2024
The neuroprotective BDNF/TrkB-FL system is compromised in Alzheimer’s disease (AD). Amyloid-beta triggers calpain-mediated TrkB-FL receptor cleavage, leading to TrkB-ICD formation, a novel intracellular toxic fragment. Biological fluids are used to pinpoint potential disease biomarkers and extracellular vesicles (EVs) are cell-specific carriers of promising pathological biomarkers. Thus, this work aimed to investigate the presence of TrkB-ICD in AD human samples and EVs. Protein and RNA was extracted from human AD post-mortem brain samples classified according to the Braak staging (stages 0-II
ePoster · Neuroscience