Irreversible shift of TDP-43 into the cytoplasm alters its protein network
Jerneja Nimac, Sonja Prpar Mihevc, Julija Mazej, Helena Motaln, Vera Župunski, Boris Rogelj · FENS Forum 2024
TDP-43 is an important DNA/RNA-binding protein that is mainly localized in the cell nucleus. In neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), it is mislocalized and becomes a central component of cytoplasmic protein aggregates. Our aim was to investigate the interactome of wild-type TDP-43 and TDP-43 without nuclear localization signal (dNLS), which mimics a pathological condition in ALS. We generated inducible mammalian cell lines expressing TDP-43wt and TDP-TDP-43dNLS. Using the BioID method, we investigated the interactome of these
ePoster · Neuroscience