Metabolic response of cortical neurons, astrocytes, and blood vessels to locomotion
Alisa Tiaglik, Ksenia Morozova, Anna Fedotova, Milena Shestopalova, Anton Zalygin, Vladimir Oleinikov, Dmitriy Bilan, Nadezda Brazhe, Alexey Semyanov · FENS Forum 2024
Neurons and astrocytes, key elements of the brain active milieu, differ in the metabolic profile and the electron transport chain (ETC) organization. We assessed the redox state of the ETC in two cell types with simultaneous analysis of blood oxygen saturation level (sO2) in awake mice with Raman microspectroscopy. For cell identification, green fluorescent protein (GFP) was expressed in astrocytes, and near-infrared protein (NirFP) was expressed in neurons following AAV injections in the somatosensory cortex (S1). To monitor H2O2, HyPer7 was expressed in the mitochondria of either astrocytes
ePoster · Neuroscience