Multidonor human cortical chimeroids reveal individual susceptibility to neurotoxic triggers
Noelia Antón-Bolaños, Irene Faravelli, Tyler Faits, Sophia Andreadis, Sebastiano Trattaro, Rahel Kastli, Xian Adiconis, Daniela J. Di Bella, Matthew Tegtmeyer, Ralda Nehme, Joshua Z. Levin, Aviv Regev, Paola Arlotta · FENS Forum 2024
Inter-individual genetic variation affects the susceptibility to many diseases. Efforts to study the molecular mechanisms mediating the impact of human genetic variation are limited. Here, we present human brain “Chimeroids”, a highly reproducible, multi-donor human brain cortical organoid model. It is created by re-aggregating neural stem cells from multiple single-cell donor organoids in a manner that allows each donor to produce all cell lineages of the cerebral cortex, even when using pluripotent stem cell lines with notable growth biases. We leveraged Chimeroids to investigate inter-indiv
ePoster · Neuroscience