Topic: Ca2+-imaging

Painful diabetic neuropathy (PDN), a chronic and often incurable syndrome, is one of the most common and unpleasant complications of diabetes. Effective clinical interventions for PDN are very limited and already developed approaches are characterized by lack of molecular or cellular target specificity and a short duration of therapeutic effects. Numerous investigations causally link upregulation of Cav3.2 T-type Ca2+ channels in peripheral nociceptive neurons to painful symptoms of PDN. Here we suggest an approach, based on implementation of virus-mediated cell-specific delivery of vectors ex

Electrical-based neuroprostheses reported promising clinical outcomes for the treatment of neurological disorders. Nevertheless, they suffer from low spatial resolution and lack of natural neurotransmission specificity that require alternative bio-mimetic stimulation strategies. Chemical-based neuroprostheses would represent a promising option to emulate the physiological synaptic communication by providing localized delivery of neurotransmitters. While various drug delivery approaches have been investigated, an efficient neurotransmitter-based neuronal stimulation at the nanoscale still remai

The medial prefrontal cortex (mPFC) is important for higher cognitive functions. In vivo recordings of neuronal activity in the mPFC have been achieved via invasive electrical and optical approaches. Recently developed three-photon (3P) in vivo imaging enables deeper tissue penetration than two-photon microscopy. Here, we demonstrate high resolution structural and functional 3P-imaging in the mPFC of the mouse at unprecedented depth. Specifically, we perform Ca2+-imaging in awake head-fixed mice up to a depth of 1700 µm in the mPFC. We record Ca2+-transients in neurons, astrocytes and longitud