The proteomic architecture of the synaptic engram supporting context memory
Biswajit Moharana, Panthea Nemat, Renee Pullen, Anna Gradl, Remco Klaassen, Cora Chadick, Rolinka van der Loo, Yvonne Gouwenberg, Frank Koopmans, Juan Garcia Vallejo, Michel van den Over, August Smit, Priyanka Rao-Ruiz · FENS Forum 2024
Sparsely distributed hippocampal CA1 engram cells, activated at the time of learning and receiving input from the CA3, are critical to contextual memory consolidation. This manifests as an augmentation of synaptic connectivity between these hippocampal subfields. Enhanced green fluorescent protein (GFP) reconstitution across synaptic partners (eGRASP) facilitates structural and electrophysiological analysis of input-specific synapses between engram cells. However, the protein landscape shaping and maintaining this enhanced engram synaptic connectivity remains unknown. Global proteomic analysis
ePoster · Neuroscience