Precise spatiotemporal photorelease of a systemically administered caged nicotine agonist in freely moving mice
Nicolas Guyon, Joachim Jehl, Sabrina Djillali Bloufa, Yasmine Layadi, Nelson Rebola, Philippe Faure, Graham Ellis-Davies, Alexandre Mourot · FENS Forum 2024
Delivering drugs to specific brain nuclei in freely moving mice presents challenges such as anatomical selectivity, slow on/off rates, and the integration of pressure-infusion with electrophysiology. While caged drugs have been widely used in brain slices for optically releasing neurotransmitters, their in vivo application has been limited. In this study, we introduce NV-epibatidine and NV-ABT594, potent photoactivatable nicotinic agonists, inactive in the dark, enabling systemic administration for local photo-release in the brain. Local photoactivation of NV-epibatidine in the ventral tegment
ePoster · Neuroscience