Topic: Calcium Imaging data Analysis

In this study, we investigate how contextual memory is shaped by observational fear learning during encoding and recall. We are imaging neurons in layer 2/3 of the retrosplenial cortex in GCaMP6s mice with two-photon Ca2+ imaging using a head-fixed observational fear learning paradigm in virtual reality. The animals are surrounded by three screens and run on a running wheel as they travel through 3 different VR environments. In two of the environments, we play recordings of mouse vocalisations emitted while they were receiving foot shocks, and a neutral sound in the third environment. A single