Functional characterization of healthy and Alzheimer’s disease-related 3D neurospheres formed using human iPSC-derived glutamatergic neurons, GABAergic neurons, and astrocytes
Oksana Sirenko, Krishna Macha, Carole Crittenden, Rebecca Fiene, Scott Schachtele, Coby Carlson, Georgy Pyatakov · FENS Forum 2024
Neural 3D organoids from human induced pluripotent stem cells (iPSC) are a rapidly developing technology with great potential for understanding brain development and neuronal diseases. A promising parallel approach is assembling structures similar to 3D spheroids or “neurospheres” by using defined combinations of fully differentiated human iPSC-derived cells in tri-culture, including glutamatergic neurons, GABAergic neurons, and astrocytes. In this study, we chose to model Alzheimer’s Disease (AD) by incorporating allelic variants of the ApoE gene (2/2, 3/4, and 4/4) to create disease-specific
ePoster · Neuroscience