Decoding neuronal identity maintenance and progenitor plasticity in extended brain organoid cultures
Irene Faravelli, Noelia Anton-Bolanos, Tyler Faits, Anqi Wei, Sophia Andreadis, Marta Montero Crespo, Yang Sung Ming, Xian Adiconis, Rahel Kastli, Bruna Paulsen, Giorgia Quadrato, Jeff Lichtman, Ed Boyden, Aviv Regev, Joshua Levin, Paola Arlotta · FENS Forum 2024
Human brain organoids offer a unique opportunity to understand developmental milestones, but we still have limited knowledge about the complex molecular mechanisms involved in long-term processes of maturation and maintenance of the tissue. In this study, we profiled brain cortical organoids cultured for periods from 180 to over 1000 days using single-cell RNA-sequencing, electron microscopy and functional assays.Our analysis revealed various neuronal populations with specific subclusters that gradually decreased in complexity over time, while astrocyte populations became more prominent. We hy
ePoster · Neuroscience