Genetic screen of cardinal interneuron function in swim and limb behavior over frog metamorphosis
Florina Alexandra Toma, David Vijatovic, Zoe P. M. Harrington, Mara J. Julseth, Christoph Sommer, Robert Hauschild, Lora B. Sweeney · FENS Forum 2024
The Xenopus laevis frog shows a wide range of motor behaviors during metamorphosis, transitioning from tail-based escape swimming, to free swimming, and finally, limb-based movement. This swim-to-limb transition allows us to study in a single organism how motor and interneurons in the spinal cord generate these behaviors. Here, utilizing advances in genetic manipulation and behavioral tracking, we dissect the molecular features and contribution of each spinal cord cardinal class to swim versus limb behavior over frog metamorphosis.We devise a high-throughput CRISPR screen, in which we knock ou
ePoster · Neuroscience