Mouse retinal rod bipolar cells express different L-type Ca channel transcripts
Elisa Roth, Hartwig Seitter, Matthias Ganglberger, Lucia Zanetti, Marta Campiglio, Georgios Fotakis, Zlatko Trajanoski, Alexandra Koschak · FENS Forum 2024
Ribbon synapses in the retina provide sustained glutamatergic transmitter release that is controlled by Cav1.4 L-type calcium channels in photoreceptors. In bipolar cells the voltage-gated calcium channels (VGCC) responsible for release are less conclusive. We therefore addressed the VGCCs expressed in mouse rod bipolar cells (RBC). We enriched RBCs by FACS-sorting and quantified mRNA expression levels of Cav1, Cav2 and auxiliary VGCC subunit isoforms, including selected splice variants. Transcript variants of candidate VGCC genes were confirmed in RT-PCR and through screening of RNAseq datase
ePoster · Neuroscience