Topic: chimeric in vitro model

Studying the dynamic role of microglia in brain development and neurodegenerative diseases requires models that closely resemble the human brain environment. While murine models provide valuable insights, they often have important transcriptional and cellular differences from humans. Addressing this, we developed a chimeric in vitro model to study microglial behavior within a human-like neuronal setting.We co-cultured human iPSC-derived human organoids (hORG) with mouse brain slice cultures (mBSC). In this chimeric setup we found multiple interactions between mouse and human tissue, markedly a