Topic: chondroitin-sulfate proteoglycans

ePoster: 2 ePosters

Human brain ECM: From morphogenetic role to specification of cortical microcircuitries

Natasa Jovanov Milosevic, Mihaela Bobic Rasonja, Marija Milkovic Perisa, Ivan Banovac, Sara Trnski Levak, Matea Drlje Curt, Andrija Štajduhar, Ivana Pogledic, Goran Sedmak, Zdravko Petanjek · FENS Forum 2024

The brain extracellular matrix (ECM) provides a physical framework and biochemical milieu for early (embryonic) morphogenetic and later (fetal and neonatal) histogenetic events. The specific ECM constituents and their dynamic spatial and temporal distribution at transcriptomics and protein levels were researched using RNAseq analysis and immunohistochemistry in 200 postmortem fetal and postnatal human brains. The ECM is especially prominent in the transiently voluminous histogenetic zones such as the neocortical preplate, subplate, and marginal zone. Still, it is also present in the smaller de

ePoster · Neuroscience

Impact of ventral hippocampal chondroitinase-ABC infusion on perineuronal nets and diffuse extracellular matrix in male Lister hooded rats

Jacob Juty, Rachel Grasmeder Allen, Jacco Renstrom, Jennifer Fletcher, Charlotte Taylor, John Gigg, Michael Harte, Tobias Bast · FENS Forum 2024

Perineuronal nets (PNNs) in the hippocampus primarily surround inhibitory interneurons. PNNs have been suggested to regulate synaptic plasticity and support GABAergic inhibition, and PNN disruption has been implicated in brain disorders, including schizophrenia. Yet, the cognitive and behavioural consequences of hippocampal PNN loss largely remain to be studied.The enzyme chondroitinase-ABC (chABC) degrades chondroitin-sulfate proteoglycans (CSPGs), a main component of PNNs and the diffuse extracellular matrix (ECM), and has been used to study PNN function in animal models.Here, we characteris

ePoster · Neuroscience