Simultaneous calcium imaging and extracellular electrophysiology using CMOS-based imaging devices with an integrated carbon electrode for freely moving mice experiments
Virgil Christian Castillo, Ryoma Okada, Kuang Chih Tso, Yoshinori Sunaga, Yasumi Ohta, Hironari Takehara, Hiroyuki Tashiro, Kiyotaka Sasagawa, Jun Ohta · FENS Forum 2024
Simultaneous recording of extracellular electrophysiological and calcium imaging signals enables a more comprehensive understanding of neural activity. Thus, we aimed to incorporate extracellular recording to our calcium imaging devices. The electrophysio-imaging device consists of a 450 x 1660 µm CMOS chip and micro-LED mounted on a flexible printed circuit substrate. The imaging device had a field of view of 900 x 300 µm composed of 120 x 90 pixels recording at 10 frames per second. An absorption filter was fixed on the imaging surface to block excitation light from the LED and allow only em
ePoster · Neuroscience