Topic: compensatory endocytosis

Mammalian synapses facilitate high-frequency sustained neurotransmission through the fusion of synaptic vesicles (SVs) with the presynaptic membrane, followed by rapid recycling of SV machinery, including SNARE components, SV proteins, and membrane lipids over milliseconds to minutes. Visualizing these SV exo-endocytosis events is challenging due to their speed and nanoscale localization. To address this, we identified candidate SV proteins with abundant representation on SVs for precise labelling, aiming to enhance contrast. Conventional methods like over-expression, antibody-based labelling,