Brain implantation of 3D constructs of the cerebral cortex generated via microfluidic printing
Luana Campos Soares, Mona Barakat, Mingyu Li, Elisa Marozzi, Gabriel Moser, Yufan Xu, Linna Zhou, Hagan Bayley, Zoltan Molnár, Francis Szele · FENS Forum 2024
Implantation and integration of human neural stem cells (hNSCs) to mouse host brains has been reported by previous studies. We are interested in generating a pre-organised construct from hNSCs that contains progenitors with different cerebral cortical layer identity using 3D printing technologies (Jin et al., Nat Commun 14, 5986 (2023). We optimised a sequential isolation protocol of lower and upper cortical layer specific hNSCs generated from human pluripotent stem cells (hPSCs). We confirmed the ability of these cells to generate neurons of the upper or lower cortical layers both in vitro an
ePoster · Neuroscience