ePoster · Neuroscience
A new transgenic mouse line for the visualization of endogenous biotinylated Neuroligin-1
Charles Ducrot, Adèle Drouet, Béatrice Tessier, Chloé Desquines, Alexandre Favereaux, Mathieu Letellier, Olivier Thoumine · FENS Forum 2024
Synapses are established and maintained by adhesion molecules, which include post-synaptic neuroligins (NLGNs). In the absence of highly specific antibodies, the sub-cellular distribution and synaptic function of NLGNs have been extensively manipulated using knock-out, over-expression or rescues approaches, leading to controversial results. In this context, we generated a new transgenic knock-in mouse strain in which endogenous NLGN1 has been N-terminally tagged with a biotin acceptor peptide (bAP) by gene editing using the CRISPR-Cas-9 strategy. Native NLGN1 can be enzymatically biotinylated