Single-cell CRISPR screening in cerebral organoids identifies developmental and cell type-specific defects of autism
Chong Li, Jonas Simon Fleck, Catarina Martins-Costa, Thomas R. Burkard, Jan Themann, Marlene Stuempflen, Angela Maria Peer, Ábel Vertesy, Jamie B. Littleboy, Cristopher Esk, Ulrich Elling, Gregor Kasprian, Nina S. Corsini, Barbara Treutlein, Juergen A. Knoblich · FENS Forum 2024
The precise generation of diverse neuronal cell types is crucial for normal brain function. Genetic mutations leading to impaired neuronal development have long been linked to neurodevelopmental disorders. Cerebral organoids can be used to investigate neurodevelopmental disorders in a human context but are limited by variability and low throughput. We have developed the CRISPR-human organoids-scRNA-seq (CHOOSE) system that utilizes verified pairs of gRNAs, inducible CRISPR/Cas9-based genetic disruption, and single-cell transcriptomics for pooled loss-of-function screening in mosaic organoids.
ePoster · Neuroscience