Topic: cryo-electron tomography

In situ cryo-electron tomography can reveal interactions between pathogens and their hosts inside intact cellular environments. This seminar uses apicomplexan parasites and gram-negative bacteria to explain the workflow from sample preparation through image acquisition and analysis. It covers freezing, cryo-focused ion beam milling, cryo-electron tomography, correlative light and electron microscopy, tomogram reconstruction, segmentation and subtomogram averaging. The presentation discusses which aspects of the host-pathogen interface these approaches can resolve, their advantages and limitati

Faculty appointments support fundamental research on molecules, cells, organisms and their interactions across scales. Areas include chemical biology, biochemistry, microbial biology, structural and molecular biology, cell biology and cryo-electron tomography, including quantitative and computational approaches. Faculty lead independent research, teach and mentor trainees. Appointments may begin in July 2027 or later by agreement.