Topic: CSF tracers

Cerebrospinal fluid (CSF) flow is crucial for brain homeostasis. Among various methods to modulate CSF flow, focused ultrasound stimulation (FUS) has been reported to enhance CSF circulation. However, real-time in vivo observations of FUS targeting the skull base are scarce. We have shown a novel method of directing FUS towards the skull base, demonstrating that it can facilitate CSF influx, as evidenced by various in vivo imaging techniques. Male C57BL/6 mice aged 8-10 weeks were intraperitoneally injected with a mixture of Zoletil and xylazine for anesthesia and exposed to the atlanto-occipi