Viral vector manipulation of neurons activated by fear learning in the centromedial amygdala
Neha Acharya, Patricia Molina Molina, Hanna Hörnberg, Jaime Fabregat Nabás, Leire Rodríguez Romero, Antonio Armario, Raul Andero · FENS Forum 2024
Neurons in the centromedial amygdala (CeM) are activated during fear memory formation. We designed a new viral vector, AAV9-pfos-hM4Di:mCherry:PEST expressing Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) under a c-fos promoter, enabling the specific manipulation of activated neurons. We use this viral vector to temporarily inactivate the neurons involved during cued-fear learning in the CeM and to uncover their molecular profile.Male and female wildtype C57BL/6J mice are injected with the viral vector in the CeM, exposing one group to the shocks paired with the tones (f
ePoster · Neuroscience