Topic: cyto-GCaMP6f

Astrocytes are an essential part of the brain active milieu and generate complex spatiotemporal patterns of calcium activity during animal behavior. The properties, mechanisms, and physiological relevance of these patterns are still poorly understood. Here, we performed two-photon calcium imaging in cortical astrocytes expressing cyto-GCaMP6f in head-fixed mice running on a rotating platform. In each imaging frame, suprathreshold calcium activity regions (segments of spatiotemporal calcium events) were detected. We analyzed ΔF/F, total active area, mean segment area, and segment density during