An in vitro evaluation on the possible role of vitamin B12 in neuronal recovery and neuronal homeostasis
Aimee Rachel Mathew, Luca Buccini, Anacleto Proietti, Giacomo Di Matteo, Luisa Mannina, Marco Rossi, Daniele Passeri, Virve Cavallucci, Marco Fidaleo · FENS Forum 2024
Some studies demonstrate the beneficial role of high doses of vitamin B12 (VitB12) supplementation for the recovery from specific neurological impairments; however, the detailed mechanisms are not clearly elucidated. With this in mind, we employed an in vitro cell model made of hydrogen peroxide-insulted differentiated-SH-SY5Y (exhibiting a neuron-like phenotype) to study the effect of both a high and the physiological dose of VitB12 during recovery. The physiological dose of VitB12 showed an improvement in the cell count (although cell viability, evaluated by MTT, was comparably decreased in
ePoster · Neuroscience