Topic: digital holography

The state-of-the-art techniques for all-optical interrogation of neuronal circuits in vivo typically combine two-photon (2P) calcium imaging and digital holography to precisely activate target neurons with sculpted light patterns. This approach is however limited in speed and in throughput, ultimately due to the intrinsic constraints of digital holography. We employed acousto-optic deflectors (AOD)-based 2P approaches (“ULoVE”, Villette et al. 2019; “3D-CASH”, Akemann et al. 2022) to simultaneously record and perturb neuronal activity in awake head-fixed mice across single planes or three-dime