The human-specific nicotinic receptor subunit CHRFAM7A (dupα7) reduces α7 nAChR function in human iPSC-derived and transgenic mouse neurons
Gokce Ilayda Gorgulu Soztekin, Vinita Jagannath, Stephanie Pons, Matthias Groszer, Uwe Maskos, Sigismund Huck, Petra Scholze · FENS Forum 2024
We investigated the impact of the human-specific protein CHRFAM7A (dupα7) on the function of α7 nicotinic acetylcholine receptors (nAChRs) in two different types of neurons: human induced pluripotent stem cell (hiPSC)-derived cortical neurons, and superior cervical ganglion (SCG) neurons, taken from transgenic mice expressing CHRFAM7A. The CHRFAM7A co-assembles with CHRNA7 but lacks a major part of the extracellular N-terminal ligand-binding domain. We assessed the function of homomeric and hybrid pentameric receptors with Fura-2 calcium imaging and three different α7-specific ligands: PNU2829
ePoster · Neuroscience