Investigation of N-acetyl spermine mechanism of action in an ex vivo model of human brain neocortex and its use as a potential antiseizure medication compound
Alice Podestà, Laura Monni, Thilo Kalbhenn, Matthias Simon, Ran Xu, Julia Onken, Ulrich-Wilhelm Thomale, Henrik Alle, Dietmar Schmitz, Martin Holtkamp, Jörg Geiger, Pawel Fidzinski · FENS Forum 2024
N-acetyl spermine (NASPM) is known from in vitro and in vivo animal models for its action as calcium permeable AMPA receptors (CP-AMPARs) antagonist. Our study aim to investigate the impact of NASPM on an ex vivo human brain slice neocortex model, focusing on its ability to suppress epileptic activity. While AMPA receptors play a central role in neocortical signaling and neuronal activity, the contribution of CP-AMPARs, particularly in the context of seizure-induced inhibition/excitation imbalance, remains unknown. By applying NASPM to ex vivo human cortex slices, we aimed to investigate its a