Topic: excitatory synaptic inputs

Dravet syndrome (DS) is a rare form of epilepsy caused by loss-of-function mutations of the voltage-gated sodium channel NaV1.1. The proposed disease mechanism suggests that these mutations compromise the ability of inhibitory interneurons to regulate network activity, leading to seizure events. However, standard treatments aimed at restoring inhibition have very limited efficacy, suggesting a potential role for excitatory neurons in DS pathogenesis. To explore this, we generated excitatory and inhibitory neurons from control and Dravet hiPSCs and combined them at physiological ratios into i