Topic: fiber autofluorescence

Intensity fiber photometry has contributed to significant advances in neuroscience over the past ten years. Recording the calcium-dependent change in fluorescence intensity enables the correlation of the mouse brain activity with different behaviors and external excitations. However, intensity, even normalized using the isosbestic excitation point of the calcium sensor, is not a reliable parameter when comparing the different brain regions or animals, as conformational changes and oscillations in the pH value modulate the signal. Also, for some compounds, there are no intensiometric sensors. I