Physiological measurements of activity and microtubule health in human iPSC-derived neurons using fluorescence and second harmonic microscopy
Natalia Garcia Perez, Emma Moles-Garcia, Milvia Alata, Pieter Vanden Berghe · FENS Forum 2024
Second harmonic imaging is a non-linear optical process that relies on the interaction of 2 photons with the sample. In this interaction, a photon of half the wavelength of the initial incoming light is generated. The generation of second harmonic signals requires the sample to have non-centrosymmetric molecular organization. Microtubules are composed of polymerized tubulin dimers and they are a major component in the cytoskeleton of neurons. Axons, in particular, have unipolarized microtubules with the plus ends growing away from the soma. This leads to an aligned microtubule organization in
ePoster · Neuroscience