Topic: fluorescence videos

Closed-loop neuroscience experimentation, where recorded neural activity is used to modify the experiment on-the-fly, is critical for deducing causal connections and optimizing experimental time. A critical step in creating a closed-loop experiment is real-time inference of neural activity from streaming recordings. One challenging modality for real-time processing is calcium imaging (CI) via multi-photon microscopy. CI enables the recording of activity in large populations of neurons however, often requires batch processing of the video data to extract single-neuron activity from the fluoresc