A novel fluorescent sensor reveals evoked chloride transients in vivo
Giacomo Pasquini, Melissa Santi, Simone Giubbolini, Andrew Beale, Marco Brondi, Irene Carrozzo, Alberto Egidi, Gabriele Nardi, Hanako Tsushima Semini, Marta Stancampiano, Anna Fassio, Silvia Landi, Claudia Lodovichi, Rachel Edgar, John O'Neill, Daniele Arosio, Gian Michele Ratto · FENS Forum 2024
Fast inhibition in the brain is mostly supported by chloride currents through the GABAA receptors. The driving force of these currents depends on the intracellular concentration of chloride ([Cl-]i) that is an important, but little studied, determinant of the inhibitory drive and of the coding performed by visual cortex. Recently, we have demonstrated a diurnal regulation of [Cl-]i that modulates the local field potential elicited by patterned visual stimuli (doi:10.1038/s41467-023-42711-7). However, the lack of a proper tool has limited the direct investigation of Cl- changes in response to s
ePoster · Neuroscience