Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide release
Xuehan Zhou, Carrie Stine, Patricia Oliveira Prada, Debora Fusca, Kevin Assoumou, Jan Dernic, Musadiq A. Bhat, Ananya S. Achanta, Joseph C. Johnson, Sanjana Jadhav, Corinna A. Bauder, Lukas Steuernagel, Luca Ravotto, Dietmar Benke, Bruno Weber, Miriam Stoeber, Peter Kloppenburg, Jens C. Brüning, Michael R. Bruchas, Tommaso Patriarchi · FENS Forum 2024
Nociceptin/orphanin-FQ (N/OFQ) is a recently appreciated critical opioid peptide with key regulatory functions in several central behavioral processes including motivation, stress, feeding, and sleep. The functional relevance of N/OFQ action in the mammalian brain remains unclear due to a lack of high-resolution approaches to detect this neuropeptide with appropriate spatial and temporal resolution. Here we develop and characterize NOPLight, a genetically encoded sensor that sensitively reports changes in endogenous N/OFQ release. We characterized the affinity, pharmacological profile, spectra