Topic: genetically encoded sensors

Recent advances in biosensor technology have paved the way for an unprecedented level of inquiry into the spatiotemporal dynamics of neurochemical release in the brain. These sensors directly and specifically report synaptic signals as they are received by their receptors. dLight is a family of dopamine sensors with broadly tuned apparent affinity and dynamic range suitable to measure dopamine release between pM and µM range. They exhibit high affinity, millisecond response times, high signal-to-noise ratio (SNR), along with expression and targeting suitable for use in vitro and in vivo to tra

For decades, the molecule Serotonin is of interest not only but also because of its various functions within the brain. While the direct measurement of serotonin release in the brain is challenging, new genetically encoded sensors for serotonin opened new possibilities to measure release of serotonin in a high temporal and spatial resolution. Here we report the newest developments of one of these genetically encoded sensors for serotonin, called sDarken.Based on the initial published sDarken (Kubitschke et al. 2023) we now utilized mutagenesis, to develop a new color shifted variant which show