Chemogenetic modulation of CX3CR1+ microglia in the intrahippocampal kainic acid mouse model of drug-resistant temporal lobe epilepsy
Jo Bossuyt, Ilse Smolders · FENS Forum 2024
Microglia seem able to alter the excitatory/inhibitory balance in temporal lobe epilepsy (TLE). Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) enable cell-specific modulation. We aim to investigate whether Gi-DREADD-based modulation of microglia reduces the amount of spontaneous recurrent seizures (SRS) in a mouse model of TLE.CX3CR1CreERT2/CreERT2 mice were crossed with R26LSL-Gi-DREADD/LSL-Gi-DREADD mice to obtain CX3CR1CreERT2/+:R26LSL-Gi-DREADD/+ and CX3CR1CreERT2/+:R26+/+ mice, as controls. Tamoxifen was administered to induce Gi-DREADD expression in CX3CR1+ cells. F
ePoster · Neuroscience