Endoplasmic reticulum quality control machinery validates structural changes, not functionality, of NMDA receptors
Marek Ladislav, Jakub Netolicky, Marharyta Kolcheva, Petra Zahumenska, Anna Misiachna, Martin Horak · FENS Forum 2024
AIMS:Investigate whether the endoplasmic reticulum (ER) quality control machinery verifies the structure of ligand-binding domains (LBDs) of NMDARs or controls the functionality of NMDARs.METHODS:HEK293 cells were used to express wild-type (WT) or mutation forms of GluN1/GluN2A and GluN1/GluN2B receptors. Surface expressions were observed using fluorescence confocal microscopy. Electrophysiological properties of NMDARs were assessed via the whole-cell patch-clamp technique.RESULTS:Using alanine substitutions combined with microscopy and electrophysiology, we found that surface expression of Gl
ePoster · Neuroscience