Topic: glutamate imaging

Dendrites play a fundamental role in neuronal computation. It is thought that functionally defined regions of dendritic branches, termed ‘dendritic computational units’ are used to decode information transmitted by synaptic input. This information is encoded by the corresponding dendritic output, to perform complex neuronal computations. To investigate dendric input-output in vivo, it is necessary to record synaptic input and dendritic output simultaneously. To do this we perform simultaneous glutamate imaging and voltage imaging from the spiny dendrites of single Purkinje neurons in awake mic