The role of disulfide bonds in GluN1 in the regulation of the early trafficking and functional properties of GluN1/GluN3A subtypes of NMDA receptors
Petra Zahumenská, Jakub Netolický, Marek Ladislav · FENS Forum 2024
AIMS: Investigate the impact of disrupting specific disulfide bonds in the GluN1 subunit on the early trafficking and functional properties of unconventional NMDARs.METHODS:HEK293 cells and rat hippocampal neurons were used to express wild-type (WT) or mutant forms of GluN1/GluN3A receptors. Surface expression and colocalization of NMDARs with the Golgi apparatus (GA) were observed using fluorescence confocal microscopy. Electrophysiological properties of NMDARs were assessed via patch-clamp technique.RESULTS:Disrupting disulfide bonds in the GluN1 subunit co-transfected with GluN3A subunit le
ePoster · Neuroscience