Aims: Excessive oxidative stress is a major factor to damage retinal pigment epithelial (RPE) cells that promotes the development of retinal degeneration. We investigated the effects of 6dS, a pigment epithelium-derived factor (PEDF) derived factor on cultured RPE cells and retina of rats treated with sodium iodate (NaIO3). Methods: Annexin V/PI staining was used to determine ARPE-19 cell viability. FerroOrange and immunostaining of acrolein were used to detect intracellular labile ion (Fe2+)-dependent cell ferroptosis and lipid peroxidation reaction in plasma membrane. Immunoblotting and real
ePoster · Neuroscience