In-vivo co-registering of functional calcium activity with nine other virally expressed fluorophores through an implanted GRIN lens
Nicolai Urban, Mary L Phillips, Ryohei Yasuda · FENS Forum 2024
Head-mounted miniscopes offer an interesting tradeoff: at the expense of image quality and versatility, they enable the correlation of animal behavior with neuronal activity without limiting free movement. Combining this with the ability to use additional fluorescent markers in-vivo, would allow us to gather further information on the observed neurons, such as genetic traits, specific cell-types, or connections to other brain regions. Unfortunately, current miniscopes cannot discriminate more than one additional fluorophore, and sacrificing the animals for post-experiment brain fixation and ex
ePoster · Neuroscience